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Fluorescence sensors development and application
Nanobiosensors development and application
Interactions between biomacromolecules and metal ions and organic small molecules
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Fluorescence sensors development and application

   发布时间:2015-08-29    作者:王志林    浏览量:613
 

The development of highly selective and sensitive fluorescent sensors has received considerable attention owing to their utility in clarifying and analyzing the roles of ions and biomolecules in living systems. A series  of water-soluble fluorescent sensors based on the pyridine-containing tetraazamacrocycle were designed and synthesized, which show high selectivity and sensitivity for ratiometric recognition Of Cu2 , Zn2 , Hg2 ,  pyrophosphate, calf thymus DNA and bovine serum albumin, respectively. Wherein, an NBD-armed tetraazamacrocyclic fluorescent probe was specifically localized in lysosomes and successfully applied to visualize Cu2  as well as to monitor Cu2  level change in the lysosomes of living cells. A Zn2  tetraazamacrocycle complex bearing three naphthalene moieties recognizes binds and causes damage to DNA, and shows considerable cytotoxicity against human cancer cell lines with a different apoptotic pathway from that of cisplatin.